Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events.

ABSTRACT:

Background

The Ccr4-Not complex is mostly known as the major eukaryotic deadenylase. However, several studies have uncovered roles of the complex, in particular of the Not subunits, unrelated to deadenylation and relevant for translation. In particular, the existence of Not condensates that regulate translation elongation dynamics has been reported. Typical studies that evaluate translation efficiency rely on soluble extracts obtained after the disruption of cells and ribosome profiling. Yet cellular mRNAs in condensates can be actively translated and may not be present in such extracts.

Results

In this work, by analyzing soluble and insoluble mRNA decay intermediates in yeast, we determine that insoluble mRNAs are enriched for ribosomes dwelling at non-optimal codons compar

SUBMITTER: Allen G 

PROVIDER: S-EPMC9940351 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature