Water channel protein AQP1 in cytoplasm is a critical factor in breast cancer local invasion.

ABSTRACT:

Background

Metastasis of breast cancer grows from the local invasion to the distant colonization. Blocking the local invasion step would be promising for breast cancer treatment. Our present study demonstrated AQP1 was a crucial target in breast cancer local invasion.

Methods

Mass spectrometry combined with bioinformatics analysis was used to identify AQP1 associated proteins ANXA2 and Rab1b. Co-immunoprecipitation, immunofluorescence assays and cell functional experiments were carried out to define the relationship among AQP1, ANXA2 and Rab1b and their re-localization in breast cancer cells. The Cox proportional hazards regression model was performed toward the identification of relevant prognostic factors. Survival curves were plotted by the Kaplan-Meier method and compare

SUBMITTER: Guo Z 

PROVIDER: S-EPMC9940370 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature