P2RX7 promotes osteosarcoma progression and glucose metabolism by enhancing c-Myc stabilization.

ABSTRACT:

Background

Osteosarcoma is the most common malignant tumor in bone and its prognosis has reached a plateau in the past few decades. Recently, metabolic reprogramming has attracted increasing attention in the field of cancer research. In our previous study, P2RX7 has been identified as an oncogene in osteosarcoma. However, whether and how P2RX7 promotes osteosarcoma growth and metastasis through metabolic reprogramming remains unexplored.

Methods

We used CRISPR/Cas9 genome editing technology to establish P2RX7 knockout cell lines. Transcriptomics and metabolomics were performed to explore metabolic reprogramming in osteosarcoma. RT-PCR, western blot and immunofluorescence analyses were used to determine gene expression related to glucose metabolism. Cell cycle and apoptosis w

SUBMITTER: Sheng G 

PROVIDER: S-EPMC9940387 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature