Engineering Saccharomyces cerevisiae for enhanced (-)-α-bisabolol production.

ABSTRACT: (-)-α-Bisabolol is naturally occurring in many plants and has great potential in health products and pharmaceuticals. However, the current extraction method from natural plants is unsustainable and cannot fulfil the increasing requirement. This study aimed to develop a sustainable strategy to enhance the biosynthesis of (-)-α-bisabolol by metabolic engineering. By introducing the heterologous gene MrBBS and weakening the competitive pathway gene ERG9, a de novo (-)-α-bisabolol biosynthesis strain was constructed that could produce 221.96 mg/L (-)-α-bisabolol. Two key genes for (-)-α-bisabolol biosynthesis, ERG20 and MrBBS, were fused by a flexible linker (GGGS)₃ under the GAL7 promoter control, and the titer was increased by 2.9-fold. Op

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC9941373 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature