Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli and Agrobacterium tumefaciens.

ABSTRACT: Broad host range (BHR) expression vector is a vital tool in molecular biology research and application. Currently, most of the plasmid vectors used in Agrobacterium spp. are binary vectors that are designed for plant transformation, and very few are designed for expressing transgenes in Agrobacterium spp. Class 1 integrons are common genetic elements that allow for the efficient capture and expression of antibiotic resistance genes, especially in Gram-negative bacteria. One of its compound promoters, PcS + P2, was used in this study and has been reported to be the strongest class 1 integron constitutive promoter; it is referred to as "integron promoter" (P _int) henceforth. Herein, we created two versions of isopropyl-d-thiogalactopyranoside (IP

SUBMITTER: Toh WK 

PROVIDER: S-EPMC9941393 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature