Murine scald models characterize the role of neutrophils and neutrophil extracellular traps in severe burns.

ABSTRACT:

Introduction

Severe burns cause unique pathophysiological alterations especially on the immune system. A murine scald model was optimized as a basis for the understanding of immunological reactions in response to heat induced injury. The understanding of the roles of neutrophil extracellular traps (NETs) and DNases will support the development of new surgical or pharmacological strategies for the therapy of severe burns.

Methods

We studied C57BL/6 mice (n=30) and employed four scalding protocols with varying exposure times to hot water. An additional scald group with a shorter observational time was generated to reduce mortality and study the very early phase of pathophysiology. At 24h or 72h, blood was drawn and tissue (wound, liver, lung, spleen) was analyzed for the prese

SUBMITTER: Elrod J 

PROVIDER: S-EPMC9941538 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature