Phosphotungstic acid-enhanced microcomputed tomography for quantitative visualization of mouse mammary gland morphology.

ABSTRACT:

Purpose

Even though current techniques provide two-dimensional (2D) imaging of the mouse mammary gland, they fail to achieve high-resolution three-dimensional (3D) reconstruction and quantification. The objective of this study is to establish and evaluate quantitative visualization of the mouse mammary epithelium through microcomputed tomography (microCT) using phosphotungstic acid (PTA) as a contrast agent.

Approach

Ex vivo microCT scan images of the mouse mammary glands were obtained following staining by PTA, whereas for quantification we adapted volumetric parameters that are used for assessing trabecular bone morphometry and can be structurally applicable in the mammary ductal system. The proposed method was validated in distinct developmental stages and upon sho

SUBMITTER: Kolokotroni A 

PROVIDER: S-EPMC9941684 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature