Obesity hormones and itaconate mediating inflammation in human colon cancer cells - Another lead to early-onset colon cancer?

ABSTRACT:

Background

Chronic inflammation is a key feature of obesity and a hallmark of colon cancer (CC). The obesity-related hormones leptin and adiponectin alter inflammatory gene profiles in cancer, but their specific role in CC is unclear. We have previously studied the effects of leptin and the macrophage-specific mediator itaconate on M2-like macrophages. This current study evaluates their effects on CC cells.

Methods

HT-29 CC cells (derived from a young patient, stage III CC) were treated with either leptin, adiponectin, 4-octyl itaconate (OI) or dimethyl itaconate (DI). Gene expression after treatment was analyzed at four time points (3, 6, 18, and 24 h).

Results

CCL22 was upregulated after treatment with adiponectin (at 18 h [FC 16.3, p < 0.001]). IL-8 expression incr

SUBMITTER: Scheurlen KM 

PROVIDER: S-EPMC9941943 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature