The detectable anti-interferon-γ autoantibodies in COVID-19 patients may be associated with disease severity.

ABSTRACT:

Background

Neutralizing anti-interferon (IFN)-γ autoantibodies are linked to adult-onset immunodeficiency and opportunistic infections.

Methods

To explore whether anti-IFN-γ autoantibodies are associated with disease severity of coronavirus disease 2019 (COVID-19), we examined the titers and functional neutralization of anti-IFN-γ autoantibodies in COVID-19 patients. In 127 COVID-19 patients and 22 healthy controls, serum titers of anti-IFN-γ autoantibodies were quantified using enzyme-linked immunosorbent assay, and the presence of autoantibodies was verified with immunoblotting assay. The neutralizing capacity against IFN-γ was evaluated with flow cytometry analysis and immunoblotting, and serum cytokines levels were determined using the MULTIPLEX platform.

Results

SUBMITTER: Chen PK 

PROVIDER: S-EPMC9942050 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature