Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics.

ABSTRACT: T cells become activated following one or multiple contacts with antigen-presenting cells. Calcium influx is a key signaling event elicited during these cellular interactions; however, it is unclear whether T cells recall and integrate calcium signals elicited during temporally separated contacts. To study the integration of calcium signals, we designed a programmable, multiplex illumination strategy for temporally patterned optogenetics (TEMPO). We found that a single round of calcium elevation was insufficient to promote nuclear factor of activated T cells (NFAT) activity and cytokine production in a T cell line. However, robust responses were detected after a second identical stimulation even when signals were separated by several hours. Our results suggest the existence of a biochemica

SUBMITTER: Corre B 

PROVIDER: S-EPMC9942117 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature