Exploring the Substrate Specificity of a Sugar Transporter with Biosensors and Cheminformatics.

ABSTRACT: Sugars will eventually be exported transporters (SWEETs) are conserved sugar transporters that play crucial roles in plant physiology and biotechnology. The genomes of flowering plants typically encode about 20 SWEET paralogs that can be classified into four clades. Clades I, II, and IV have been reported to favor hexoses, while clade III SWEETs prefer sucrose. However, the molecular features of substrates required for recognition by members of this family have not been investigated in detail. Here, we show that SweetTrac1, a previously reported biosensor constructed from the Clade I Arabidopsis thaliana SWEET1, can provide insight into the structural requirements for substrate recognition. The biosensor translates substrate binding to the transporter into a change in fluorescence,

SUBMITTER: Park J 

PROVIDER: S-EPMC9942192 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature