Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into Multiple Gut Pathogens.

ABSTRACT: Bacteriophages can be reprogrammed to deliver antimicrobials for therapeutic and biocontrol purposes and are a promising alternative treatment to antimicrobial-resistant bacteria. Here, we developed a bacteriophage P4 cosmid system for the delivery of a Cas9 antimicrobial into clinically relevant human gut pathogens Shigella flexneri and Escherichia coli O157:H7. Our P4 cosmid design produces a high titer of cosmid-transducing units without contamination by a helper phage. Further, we demonstrate that genetic engineering of the phage tail fiber improves the transduction efficiency of cosmid DNA in S. flexneri M90T as well as allows recognition of a nonnative host, E. coli O157:H7. We show that the transducing units with the chimeric tails enhanced the overall Ca

SUBMITTER: Fa-Arun J 

PROVIDER: S-EPMC9942202 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature