Signal Peptide Efficiency: From High-Throughput Data to Prediction and Explanation.

ABSTRACT: The passage of proteins across biological membranes via the general secretory (Sec) pathway is a universally conserved process with critical functions in cell physiology and important industrial applications. Proteins are directed into the Sec pathway by a signal peptide at their N-terminus. Estimating the impact of physicochemical signal peptide features on protein secretion levels has not been achieved so far, partially due to the extreme sequence variability of signal peptides. To elucidate relevant features of the signal peptide sequence that influence secretion efficiency, an evaluation of ∼12,000 different designed signal peptides was performed using a novel miniaturized high-throughput assay. The results were used to train a machine learning model, and a post-hoc explanation of the

SUBMITTER: Grasso S 

PROVIDER: S-EPMC9942255 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature