Use of the particle agglutination/particle agglutination inhibition test for antigenic analysis of SARS-CoV-2.

ABSTRACT:

Background

The antigenicity of SARS-CoV-2 is a critical issue for the effectiveness of the vaccine, and thus, it should be phenotypically evaluated by serological assays as new field isolates emerge. The hemagglutination/hemagglutination inhibition (HA/HI) tests are well known as a representative method for antigenic analysis of influenza viruses, but SARS-CoV-2 does not agglutinate human or guinea pig red blood cells. Therefore, the antigenic analysis requires complicated cell-based assays using special equipment such as plate reader or ELISPOT analyzer.

Methods

Based on the HA/HI tests for influenza viruses, we developed the particle agglutination/particle agglutination inhibition (PA/PAI) test to easily and rapidly quantify the virus and antibody using human angiotensin-c

SUBMITTER: Kobayashi J 

PROVIDER: S-EPMC9942275 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature