Genotyping Approach to Predict Coa and Cob Antigens in Thai Blood Donor Populations

ABSTRACT:

Purpose

Coa and Cob antigens of the Colton (CO) blood group system are implicated in acute and delayed hemolytic transfusion reactions (HTRs). Owing to the inadequate supply of specific antiserum, data on CO phenotypes remain limited. This study aimed to develop genotyping methods to predict Coa and Cob antigens and to estimate transfusion-induced alloimmunization risks in three Thai blood donor populations.

Materials and Methods

The study included 2451 blood samples from unrelated healthy Thai blood donors obtained from central, northern, and southern Thailand. DNA sequencing was used to determine the CO*A and CO*B alleles. In-house PCR with sequence-specific primers (PCR-SSP) and high-resolution melting curve (HRM) assays were performed and genotyping results were compa

SUBMITTER: Nathalang O 

PROVIDER: S-EPMC9942508 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature