Pseudouridine and N1-Methylpseudouridine Display pH-Independent Reaction Rates with Bisulfite Yielding Ribose Adducts.

ABSTRACT: In RNA, pseudouridine (Ψ) and 5-methylcytidine (m⁵C) are located by their differential reactions with NaHSO₃ at pH 5. The pyrimidines were allowed to react with NaHSO₃, NaN₃, NaCN, or NaSCN at pH 5 to find that NaHSO₃ was unique in achieving quantitative yields. Pseudouridine reaction selectivity with NaHSO₃ was found at pH 7 supported by the reaction rate constants. The Ψ derivative N1-methylpseudouridine found in mRNA vaccines reacts similarly with bisulfite to yield ribose adducts.

SUBMITTER: Fleming AM 

PROVIDER: S-EPMC9942683 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature