Genome-wide analysis of heat stress-stimulated transposon mobility in the human fungal pathogen <i>Cryptococcus deneoformans</i>.

ABSTRACT: We recently reported transposon mutagenesis as a significant driver of spontaneous mutations in the human fungal pathogen Cryptococcus deneoformans during murine infection. Mutations caused by transposable element (TE) insertion into reporter genes were dramatically elevated at high temperatures (37° vs. 30°) in vitro, suggesting that heat stress stimulates TE mobility in the Cryptococcus genome. To explore the genome-wide impact of TE mobilization, we generated transposon accumulation lines by in vitro passage of C. deneoformans strain XL280α for multiple generations at both 30° and at the host-relevant temperature of 37°. Utilizing whole-genome sequencing, we identified native TE copies and mapped multiple de novo TE insertions in these lines. Movements of the T1 DNA

SUBMITTER: Gusa A 

PROVIDER: S-EPMC9942834 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature