Mitotic chromosome condensation resets chromatin to safeguard transcriptional homeostasis during interphase.

ABSTRACT: Mitotic entry correlates with the condensation of the chromosomes, changes in histone modifications, exclusion of transcription factors from DNA, and the broad downregulation of transcription. However, whether mitotic condensation influences transcription in the subsequent interphase is unknown. Here, we show that preventing one chromosome to condense during mitosis causes it to fail resetting of transcription. Rather, in the following interphase, the affected chromosome contains unusually high levels of the transcription machinery, resulting in abnormally high expression levels of genes in cis, including various transcription factors. This subsequently causes the activation of inducible transcriptional programs in trans, such as the GAL genes, even in the absence of t

SUBMITTER: Ramos-Alonso L 

PROVIDER: S-EPMC9942888 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature