Single-read tRNA-seq analysis reveals coordination of tRNA modification and aminoacylation and fragmentation.

ABSTRACT: Transfer RNA (tRNA) utilizes multiple properties of abundance, modification, and aminoacylation in translational regulation. These properties were typically studied one-by-one; however, recent advance in high throughput tRNA sequencing enables their simultaneous assessment in the same sequencing data. How these properties are coordinated at the transcriptome level is an open question. Here, we develop a single-read tRNA analysis pipeline that takes advantage of the pseudo single-molecule nature of tRNA sequencing in NGS libraries. tRNAs are short enough that a single NGS read can represent one tRNA molecule, and can simultaneously report on the status of multiple modifications, aminoacylation, and fragmentation of each molecule. We find correlations among modification-modification, modific

SUBMITTER: Hernandez-Alias X 

PROVIDER: S-EPMC9943672 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature