Identification and heterologous expression of the globomycin biosynthetic gene cluster.

ABSTRACT: Globomycin is a cyclic lipodepsipeptide originally isolated from several Streptomyces species which displays strong and selective antibacterial activity against Gram-negative pathogens. Its mode of action is based on the competitive inhibition of the lipoprotein signal peptidase II (LspA), which is absent in eukaryotes and considered an attractive target for the development of new antibiotics. Despite its interesting biological properties, the gene cluster encoding its biosynthesis has not yet been identified. In this study we employed a genome-mining approach in the globomycin-producing Streptomyces sp. CA-278952 to identify a candidate gene cluster responsible for its biosynthesis. A null mutant was constructed using CRISPR base editing where production was abolished, stron

SUBMITTER: Oves-Costales D 

PROVIDER: S-EPMC9943842 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature