Effects of methylglyoxal on shrimp tropomyosin structure and allergenicity during thermal processing

ABSTRACT: Highlights • MGO induced structural changes of tropomyosin (TM) during thermal processing.• Lys, Arg, Asp, and Gln residues of TM were modified by MGO treatment.• TM-MGO can reduce the mediator and cytokine releases in RBL-2H3 cells.• MGO decreased TM-specific IgE/IgG1, histamine and mMCP-1 levels in vivo.• MGO caused changes in TM allergic epitopes resulting in lower allergenicity. This study aimed to analyze the effect of methylglyoxal (MGO) on the structure and allergenicity of shrimp tropomyosin (TM) during thermal processing. The structural changes were determined by SDS-PAGE, intrinsic fluorescence, circular dichroism, and HPLC-MS/MS. The allergenicity was evaluated by in vitro and in vivo experiments. MGO could cause conformational structural changes in TM during thermal processing.

SUBMITTER: Yang Q 

PROVIDER: S-EPMC9943847 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature