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CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice.

ABSTRACT: In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles at genomic, transcriptomic, and protein levels. This strategy simplifies the process of generating mice with conditional alleles. For complete details on the use and execution of this protocol, please refer to Cassidy et al. (2022).¹.

SUBMITTER: Cassidy A

PROVIDER: S-EPMC9943854 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice.

Cassidy Annelise A Pelletier Stephane S

STAR protocols 20230209 1

In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles at genomic, transcriptomic, and protein levels. This strategy simplifies the process of generating mice with conditional alleles. For complete details on the use and execution of this protocol, please ...[more]

PMID: 36853660

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One-step generation of a conditional allele in mice using a short artificial intron.

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