Non-Specific Signal Peptidase Processing of Extracellular Proteins in Staphylococcus aureus N315.

ABSTRACT: Staphylococcus aureus is one of the major community-acquired human pathogens, with growing multidrug-resistance, leading to a major threat of more prevalent infections to humans. A variety of virulence factors and toxic proteins are secreted during infection via the general secretory (Sec) pathway, which requires an N-terminal signal peptide to be cleaved from the N-terminus of the protein. This N-terminal signal peptide is recognized and processed by a type I signal peptidase (SPase). SPase-mediated signal peptide processing is the crucial step in the pathogenicity of S. aureus. In the present study, the SPase-mediated N-terminal protein processing and their cleavage specificity were evaluated using a combination of N-terminal amidination bottom-up and top-down proteomics-ba

SUBMITTER: Misal SA 

PROVIDER: S-EPMC9944065 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature