A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva.
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ABSTRACT:
SUBMITTER: Wagner K
PROVIDER: S-EPMC9944410 | biostudies-literature | 2023 Feb
REPOSITORIES: biostudies-literature
Publications
A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva.
Wagner Kaitlyn K Fox Phil P Gordon Elizabeth E Hahn Westen W Olsen Kenzie K Markham Alex A Buglewicz Dylan D Selemenakis Platon P Lessard Avery A Goldstein Daniella D Threatt Alissa A Davis Luke L Miller-Dawson Jake J Stockett Halie H Sanders Hailey H Rugh Kristin K Turner Houston H Remias Michelle M Williams Maggie M Chavez Jorge J Galindo Gabriel G Cialek Charlotte C Koch Amanda A Fout Alex A Fosdick Bailey B Broeckling Bettina B Zabel Mark D MD
Scientific reports 20230222 1
In response to the SARS-CoV-2 pandemic, we developed a multiplexed, paired-pool droplet digital PCR (MP4) screening assay. Key features of our assay are the use of minimally processed saliva, 8-sample paired pools, and reverse-transcription droplet digital PCR (RT-ddPCR) targeting the SARS-CoV-2 nucleocapsid gene. The limit of detection was determined to be 2 and 12 copies per µl for individual and pooled samples, respectively. Using the MP4 assay, we routinely processed over 1,000 samples a day ...[more]
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