Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy.

ABSTRACT:

Introduction

Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers.

Material and methods

Tear films were collected on Schirmer strips from 32 canine patients (12 diabetic dogs without changes in the retina, 8 diabetic dogs with signs of DR, and 12 control dogs). Two-dimensional electrophoresis was used to separate tear film proteins prior to their identification with matrix-assisted laser desorption/ionisation-tandem time-of-flight mass spectrometry and interrogation of protein function databases to find matches.

Results

Five significantly differentially expressed proteins were identified; of those, one was

SUBMITTER: Winiarczyk D 

PROVIDER: S-EPMC9944995 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature