Porcine fungal mock community analyses: Implications for mycobiome investigations.

ABSTRACT:

Introduction

The gut microbiome is an integral partner in host health and plays a role in immune development, altered nutrition, and pathogen prevention. The mycobiome (fungal microbiome) is considered part of the rare biosphere but is still a critical component in health. Next generation sequencing has improved our understanding of fungi in the gut, but methodological challenges remain. Biases are introduced during DNA isolation, primer design and choice, polymerase selection, sequencing platform selection, and data analyses, as fungal reference databases are often incomplete or contain erroneous sequences.

Methods

Here, we compared the accuracy of taxonomic identifications and abundances from mycobiome analyses which vary among three commonly selected target gene regions (

SUBMITTER: Arfken AM 

PROVIDER: S-EPMC9945231 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature