FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma.

ABSTRACT:

Background

This research aimed to investigate the roles of fanconi anemia complementation group D2 (FANCD2) on the regulation of ferroptosis in osteosarcoma progression.

Methods

The function of FANCD2 on cell viability, invasion, migration, and tumor growth were explored. FANCD2 and pathway-related genes were determined by western blot. Ferroptosis-associated markers were determined, including lipid peroxidation, labile iron pool (LIP), ferrous iron (Fe²⁺), and ferroptosis-related genes.

Results

FANCD2 expression was increased in osteosarcoma cells. FANCD2 knockdown reduced cell viability, invasion, and migration of osteosarcoma cells. FANCD2 knockdown regulated ferroptosis-related gene expression, and distinctly increased the levels of LIP, Fe²⁺

SUBMITTER: Li X 

PROVIDER: S-EPMC9945409 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature