Proteomics-based determination of double-stranded RNA interactome reveals known and new factors involved in Sindbis virus infection.

ABSTRACT: Viruses are obligate intracellular parasites, which depend on the host cellular machineries to replicate their genome and complete their infectious cycle. Long double-stranded (ds)RNA is a common viral by-product originating during RNA virus replication and is universally sensed as a danger signal to trigger the antiviral response. As a result, viruses hide dsRNA intermediates into viral replication factories and have evolved strategies to hijack cellular proteins for their benefit. The characterization of the host factors associated with viral dsRNA and involved in viral replication remains a major challenge to develop new antiviral drugs against RNA viruses. Here, we performed anti-dsRNA immunoprecipitation followed by mass spectrometry analysis to fully characterize the dsRNA interactom

SUBMITTER: Girardi E 

PROVIDER: S-EPMC9945444 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature