Anti-σ²⁸ Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola.

ABSTRACT: FlgM, an antagonist of FliA (also known as σ²⁸), inhibits transcription of bacterial class 3 flagellar genes. It does so primarily through binding to free σ²⁸ to prevent it from forming a complex with core RNA polymerase. We recently identified an FliA homolog (FliA_Td) in the oral spirochete Treponema denticola; however, its antagonist FlgM remained uncharacterized. Herein, we provide several lines of evidence that TDE0201 functions as an antagonist of FliA_Td. TDE0201 is structurally similar to FlgM proteins, although its sequence is not conserved. Heterologous expression of TDE0201 in Escherichia coli inhibits its flagellin gene expression and motility. Biochemical and mutational analyses demonstrate that TDE0201 binds to FliA_Td and p

SUBMITTER: Kurniyati K 

PROVIDER: S-EPMC9945498 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature