The compact integration of a cascaded HCR circuit for highly reliable cancer cell discrimination.

ABSTRACT: The accurate identification of multiple biomarkers involved in disease plays a vital role in effectively distinguishing cancer cells from normal cells, facilitating reliable cancer diagnosis. Motivated by this knowledge, we have engineered a compact and clamped cascaded DNA circuit for specifically discriminating cancer cells from normal cells via the amplified multi-microRNA imaging strategy. The proposed DNA circuit combines the traditional cascaded DNA circuit with multiply localized responsive character through the elaboration of two super-hairpin reactants, thus concurrently streamlining the circuit components and realizing localization-intensified cascaded signal amplification. In parallel, the multiple microRNA-stimulated sequential activations of the compact circuit, combine

SUBMITTER: Dong P 

PROVIDER: S-EPMC9945511 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature