Deciphering the immunopeptidome in vivo reveals new tumour antigens.

ABSTRACT: Immunosurveillance of cancer requires the presentation of peptide antigens on major histocompatibility complex class I (MHC-I) molecules^1-5. Current approaches to profiling of MHC-I-associated peptides, collectively known as the immunopeptidome, are limited to in vitro investigation or bulk tumour lysates, which limits our understanding of cancer-specific patterns of antigen presentation in vivo⁶. To overcome these limitations, we engineered an inducible affinity tag into the mouse MHC-I gene (H2-K1) and targeted this allele to the Kras^LSL-G12D/+Trp53^fl/fl mouse model (KP/K^bStrep)⁷. This approach enabled us to precisely isolate MHC-I peptides from autochthonous pancreatic ductal adenocarcinoma and from lung adenocarcinoma (L

SUBMITTER: Jaeger AM 

PROVIDER: S-EPMC9945857 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature