Increased vesicle fusion competence underlies long-term potentiation at hippocampal mossy fiber synapses.

ABSTRACT: Presynaptic long-term potentiation (LTP) is thought to play an important role in learning and memory. However, the underlying mechanism remains elusive because of the difficulty of direct recording during LTP. Hippocampal mossy fiber synapses exhibit pronounced LTP of transmitter release after tetanic stimulation and have been used as a model of presynaptic LTP. Here, we induced LTP by optogenetic tools and applied direct presynaptic patch-clamp recordings. The action potential waveform and evoked presynaptic Ca²⁺ currents remained unchanged after LTP induction. Membrane capacitance measurements suggested higher release probability of synaptic vesicles without changing the number of release-ready vesicles after LTP induction. Synaptic vesicle replenishment was also enhanced. Fur

SUBMITTER: Fukaya R 

PROVIDER: S-EPMC9946361 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature