Benzo[a]pyrene toxicokinetics in humans following dietary supplementation with 3,3'-diindolylmethane (DIM) or Brussels sprouts.

ABSTRACT: Utilizing the atto-zeptomole sensitivity of UPLC-accelerator mass spectrometry (UPLC-AMS), we previously demonstrated significant first-pass metabolism following escalating (25-250 ng) oral micro-dosing in humans of [¹⁴C]-benzo[a]pyrene ([¹⁴C]-BaP). The present study examines the potential for supplementation with Brussels sprouts (BS) or 3,3'-diindolylmethane (DIM) to alter plasma levels of [¹⁴C]-BaP and metabolites over a 48-h period following micro-dosing with 50 ng (5.4 nCi) [¹⁴C]-BaP. Volunteers were dosed with [¹⁴C]-BaP following fourteen days on a cruciferous vegetable restricted diet, or the same diet supplemented for seven days with 50 g of BS or 300 mg of BR-DIM® prior to dosing. BS or DIM reduced total [¹⁴C] recov

SUBMITTER: Vermillion Maier ML 

PROVIDER: S-EPMC9946811 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature