The spatio-temporal distribution of alkaline phosphatase activity and phoD gene abundance and diversity in sediment of Sancha Lake.

ABSTRACT: The bacterial phoD gene encoding alkaline phosphatase (ALP) plays an important role in the release of soluble reactive phosphorus (SRP) from organic phosphorus in ecosystems. However, phoD gene diversity and abundance in ecosystems is poorly understood. In the present study, we sampled the surface sediments and the overlying water of Sancha Lake at 9 different sampling sites, a typical eutrophic sub-deep freshwater lake in China, in April 15 (spring) and November 3 (autumn), 2017. High-throughput sequencing and qPCR were performed to analyze the diversity and abundance of the bacterial phoD gene in the sediments. We further discussed the relationships between the diversity and abundance of the phoD gene and environmental factors and ALP activity. A total of 881,717 valid sequences were obt

SUBMITTER: Li Y 

PROVIDER: S-EPMC9946943 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature