Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression.

ABSTRACT:

Aims/hypothesis

Genome-wide studies have uncovered multiple independent signals at the RREB1 locus associated with altered type 2 diabetes risk and related glycaemic traits. However, little is known about the function of the zinc finger transcription factor Ras-responsive element binding protein 1 (RREB1) in glucose homeostasis or how changes in its expression and/or function influence diabetes risk.

Methods

A zebrafish model lacking rreb1a and rreb1b was used to study the effect of RREB1 loss in vivo. Using transcriptomic and cellular phenotyping of a human beta cell model (EndoC-βH1) and human induced pluripotent stem cell (hiPSC)-derived beta-like cells, we investigated how loss of RREB1 expression and activity affects pancreatic endocrine cell development and function. E

SUBMITTER: Mattis KK 

PROVIDER: S-EPMC9947029 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature