Rgp1 contributes to craniofacial cartilage development and Rab8a-mediated collagen II secretion.

ABSTRACT: Rgp1 was previously identified as a component of a guanine nucleotide exchange factor (GEF) complex to activate Rab6a-mediated trafficking events in and around the Golgi. While the role of Rgp1 in protein trafficking has been examined in vitro and in yeast, the role of Rgp1 during vertebrate embryogenesis and protein trafficking in vivo is unknown. Using genetic, CRISPR-induced zebrafish mutants for Rgp1 loss-of-function, we found that Rgp1 is required for craniofacial cartilage development. Within live rgp1^-/- craniofacial chondrocytes, we observed altered movements of Rab6a⁺ vesicular compartments, consistent with a conserved mechanism described in vitro. Using transmission electron microscopy (TEM) and immunofluorescence analyses, we sho

SUBMITTER: Ritter DJ 

PROVIDER: S-EPMC9947155 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature