Non-invasive monitoring of T cell differentiation through Raman spectroscopy.

ABSTRACT: The monitoring of dynamic cellular behaviors remains a technical challenge for most established techniques used nowadays for single-cell analysis, as most of them are either destructive, or rely on labels that can affect the long-term functions of cells. We employ here label-free optical techniques to non-invasively monitor the changes that occur in murine naive T cells upon activation and subsequent differentiation into effector cells. Based on spontaneous Raman single-cell spectra, we develop statistical models that allow the detection of activation, and employ non-linear projection methods to delineate the changes occurring over a several day period spanning early differentiation. We show that these label-free results have very high correlation with known surface markers of activation a

SUBMITTER: Pavillon N 

PROVIDER: S-EPMC9947172 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature