Enhancing the thermotolerance and erythritol production of Yarrowia lipolytica by introducing heat-resistant devices.

ABSTRACT: Yarrowia lipolytica has been widely used in the food biotech-related industry, where it plays the host's role in producing erythritol. Nevertheless, a temperature of about 28°C-30°C has been estimated as the yeast's optimal growth temperature, leading to the consumption of a considerable quantity of cooling water, especially in summer, which is obligatory for fermentation. Herein is described a method for improving the thermotolerance and erythritol production efficiency at high temperatures of Y. lipolytica. Through screening and testing different heat resistant devices, eight refactored engineered strains showed better growth at higher temperature and the antioxidant properties of the eight engineered strains were also improved. In addition, the erythritol titer, yield and

SUBMITTER: Liang P 

PROVIDER: S-EPMC9947466 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature