Dataset Information

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production.

ABSTRACT:

Background

Energy is the basis and assurance for a world's stable development; however, as traditional non-renewable energy sources deplete, the development and study of renewable clean energy have emerged. Using microalgae as a carbon source for anaerobic bacteria to generate biohydrogen is a clean energy generation system that both local and global peers see as promising.

Results

Klebsiella pneumonia, Enterobacter cloacae, and their coculture were used to synthesize biohydrogen using Oscillatoria acuminata biomass via dark fermentation. The total carbohydrate content in O. acuminata was 237.39 mg/L. To enhance the content of fermentable reducing sugars, thermochemical, biological, and biological with magnesium zinc ferrite nanoparticles (Mg-Zn Fe₂O₄-NPs) pretreatments were applied. Crude hydrolytic enzymes extracted from Trichoderma harzianum of biological pretreatment were enhanced by Mg-Zn Fe₂O₄-NPs and significantly increased reducing sugars (230.48 mg/g) four times than thermochemical pretreatment (45.34 mg/g). K. pneumonia demonstrated a greater accumulated hydrogen level (1022 mLH₂/L) than E. cloacae (813 mLH₂/L), while their coculture showed superior results (1520 mLH₂/L) and shortened the production time to 48 h instead of 72 h in single culture pretreatments. Biological pretreatment + Mg-Zn Fe₂O₄ NPs using coculture significantly stimulated hydrogen yield (3254 mLH₂/L), hydrogen efficiency)216.9 mL H₂/g reducing sugar( and hydrogen production rate (67.7 mL/L/h) to the maximum among all pretreatments.

Conclusion

These results confirm the effectiveness of biological treatments + Mg-Zn Fe₂O₄-NPs and coculture dark fermentation in upregulating biohydrogen production.

SUBMITTER: El-Sheekh M

PROVIDER: S-EPMC9948338 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Publications

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production.

El-Sheekh Mostafa M Elshobary Mostafa M Abdullah Eman E Abdel-Basset Refat R Metwally Metwally M

Microbial cell factories 20230222 1

<h4>Background</h4>Energy is the basis and assurance for a world's stable development; however, as traditional non-renewable energy sources deplete, the development and study of renewable clean energy have emerged. Using microalgae as a carbon source for anaerobic bacteria to generate biohydrogen is a clean energy generation system that both local and global peers see as promising.<h4>Results</h4>Klebsiella pneumonia, Enterobacter cloacae, and their coculture were used to synthesize biohydrogen ...[more]

PMID: 36814252

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Project description:Thermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the interest of studying interactions between inorganic materials (adsorbents, conductive and others) and anaerobic bacteria, the biological processes were amended with variable amounts of a zeolite type-13X in the range of zeolite/inoculum (in VS) ratios (Z/I) of 0.065-0.26 g g-1. In the batch assays, the presence of the zeolite was beneficial to increase the hydrogen titer by 15-21% with C5 and C6-sugars as compared to the control, and an increase of 27% was observed in the batch fermentation of Sargassum sp. Hydrogen yields also increased by 10-26% with sugars in the presence of the zeolite. The rate of hydrogen production increased linearly with the Z/I ratios in the experiments with C5 and C6-sugars. In the batch assay with Sargassum sp., there was an optimum value of Z/I of 0.13 g g-1 where the H2 production rate observed was the highest, although all values were in a narrow range between 3.21 and 4.19 mmol L-1 day-1. The positive effect of the zeolite was also observed in a continuous high-rate reactor fed with C5 and C6-sugars. The increase of the organic loading rate (OLR) from 8.8 to 17.6 kg m-3 day-1 of COD led to lower hydrogen production rates but, upon zeolite addition (0.26 g g-1 VS inoculum), the hydrogen production increased significantly from 143 to 413 mL L-1 day-1. Interestingly, the presence of zeolite in the continuous operation had a remarkable impact in the microbial community and in the profile of fermentation products. The effect of zeolite could be related to several properties, including the porous structure and the associated surface area available for bacterial adhesion, potential release of trace elements, ion-exchanger capacity or ability to adsorb different compounds (i.e. protons). The observations opens novel perspectives and will stimulate further research not only in biohydrogen production, but broadly in the field of interactions between bacteria and inorganic materials.

Dataset Information

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production.

Background

Results

Conclusion

Publications

Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production.

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