Peripheral immune cell profiling of double-hit lymphoma by mass cytometry.

ABSTRACT:

Background

Double-hit or Triple-hit lymphoma (DHL/THL) is a subset of high-grade B cell lymphoma harboring rearrangements of MYC and BCL2 and/or BCL6, and usually associate with aggressive profile, while current therapies tend to provide poor clinical outcomes and eventually relapsed. Further explorations of DHL at cellular and molecular levels are in demand to offer guidance for clinical activity.

Methods

We collected the peripheral blood of DHL patients and diffused large B cell lymphoma (DLBCL) patients from single institute and converted them into PBMC samples. Mass cytometry was then performed to characterize these samples by 42 antibody markers with samples of healthy people as control. We divided the immune cell subtypes based on the expression profile of surface anti

SUBMITTER: Lei T 

PROVIDER: S-EPMC9948356 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature