Companion diagnostic requirements for spatial biology using multiplex immunofluorescence and multispectral imaging.

ABSTRACT: Immunohistochemistry has long been held as the gold standard for understanding the expression patterns of therapeutically relevant proteins to identify prognostic and predictive biomarkers. Patient selection for targeted therapy in oncology has successfully relied upon standard microscopy-based methodologies, such as single-marker brightfield chromogenic immunohistochemistry. As promising as these results are, the analysis of one protein, with few exceptions, no longer provides enough information to draw effective conclusions about the probability of treatment response. More multifaceted scientific queries have driven the development of high-throughput and high-order technologies to interrogate biomarker expression patterns and spatial interactions between cell phenotypes in the tumor micr

SUBMITTER: Locke D 

PROVIDER: S-EPMC9948403 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature