A 3D human co-culture to model neuron-astrocyte interactions in tauopathies.

ABSTRACT:

Background

Intraneuronal tau aggregation is the major pathological hallmark of neurodegenerative tauopathies. It is now generally acknowledged that tau aggregation also affects astrocytes in a cell non-autonomous manner. However, mechanisms involved are unclear, partly because of the lack of models that reflect the situation in the human tauopathy brain. To accurately model neuron-astrocyte interaction in tauopathies, there is a need for a model that contains both human neurons and human astrocytes, intraneuronal tau pathology and mimics the three-dimensional architecture of the brain.

Results

Here we established a novel 100-200 µm thick 3D human neuron/astrocyte co-culture model of tau pathology, comprising homogenous populations of hiPSC-derived neurons and primary human a

SUBMITTER: Batenburg KL 

PROVIDER: S-EPMC9948470 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature