Effects of Variation in Urine Sample Storage Conditions on 16S Urogenital Microbiome Analyses.

ABSTRACT: Replicability is a well-established challenge in microbiome research with a variety of contributing factors at all stages, from sample collection to code execution. Here, we focus on voided urine sample storage conditions for urogenital microbiome analysis. Using urine samples collected from 10 adult females, we investigated the microbiome preservation efficacy of AssayAssure Genelock (Genelock), compared with no preservative, under different temperature conditions. We varied temperature over 48 h in order to examine the impact of conditions samples may experience with home voided urine collection and shipping to a central biorepository. The following common lab and shipping conditions were investigated: -20°C, ambient temperature, 4°C, freeze-thaw cycle, and heat cycle. At 48 h, all sampl

SUBMITTER: Kumar T 

PROVIDER: S-EPMC9948722 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature