Functional imaging through scattering medium via fluorescence speckle demixing and localization.

ABSTRACT: Recently, fluorescence-based optical techniques have emerged as a powerful tool to probe information in the mammalian brain. However, tissue heterogeneities prevent clear imaging of deep neuron bodies due to light scattering. While several up-to-date approaches based on ballistic light allow to retrieve information at shallow depths inside the brain, non-invasive localization and functional imaging at depth still remains a challenge. It was recently shown that functional signals from time-varying fluorescent emitters located behind scattering samples could be retrieved by using a matrix factorization algorithm. Here we show that the seemingly information-less, low-contrast fluorescent speckle patterns recovered by the algorithm can be used to locate each individual emitter, even in the pre

SUBMITTER: Soldevila F 

PROVIDER: S-EPMC9949161 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature