Functional significance of PUF partnerships in C. elegans germline stem cells.

ABSTRACT: PUF RNA-binding proteins are conserved stem cell regulators. Four PUF proteins govern self-renewal of C. elegans germline stem cells together with two intrinsically disordered proteins, LST-1 and SYGL-1. Based on yeast two-hybrid results, we proposed a composite self-renewal hub in the stem cell regulatory network, with eight PUF partnerships and extensive redundancy. Here, we investigate LST-1-PUF and SYGL-1-PUF partnerships and their molecular activities in their natural context - nematode stem cells. We confirm LST-1-PUF partnerships and their specificity to self-renewal PUFs by co-immunoprecipitation and show that an LST-1(A ^m B ^m ) mutant defective for PUF-interacting motifs does not complex with PUFs in nematodes. LST-1(A ^m B ^m ) is

SUBMITTER: Ferdous AS 

PROVIDER: S-EPMC9949348 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature