Design of a Flexible, Zn-Selective Protein Scaffold that Displays Anti-Irving-Williams Behavior.

ABSTRACT: Selective metal binding is a key requirement not only for the functions of natural metalloproteins but also for the potential applications of artificial metalloproteins in heterogeneous environments such as cells and environmental samples. The selection of transition-metal ions through protein design can, in principle, be achieved through the appropriate choice and the precise positioning of amino acids that comprise the primary metal coordination sphere. However, this task is made difficult by the intrinsic flexibility of proteins and the fact that protein design approaches generally lack the sub-Å precision required for the steric selection of metal ions. We recently introduced a flexible/probabilistic protein design strategy (MASCoT) that allows metal ions to search for optimal coordina

SUBMITTER: Choi TS 

PROVIDER: S-EPMC9949983 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature