Novel p22 and p30 dual-proteins combination based indirect ELISA for detecting antibodies against African swine fever virus.

ABSTRACT:

Introduction

African swine fever virus (ASFV) infection is one of the most complex and fatal hemorrhagic viral diseases, causing a devastating loss to the swine industry. Since no effective vaccine is available, prevention and control of ASFV heavily depends on early diagnostic detection.

Methods

In this study, a novel indirect ELISA was established for detecting antibodies against ASFV using dual-proteins, p22 and p30. Recombinants p22 and p30 were expressed and purified from E.coli vector system by recombined plasmids pET-KP177R and pET-CP204L. p22 and p30 were mixed as antigens for developing the indirect ELISA.

Results

Through optimizing coating concentrations of p30 and p22, coating ratio (p30: p22 = 1:3), and serum dilution (as 1:600), the established ELI

SUBMITTER: Li J 

PROVIDER: S-EPMC9950402 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature