Comparative analysis of the cardiomyocyte differentiation potential of induced pluripotent stem cells reprogrammed from human atrial or ventricular fibroblasts.

ABSTRACT: Background: We had shown that cardiomyocytes (CMs) were more efficiently differentiated from human induced pluripotent stem cells (hiPSCs) when the hiPSCs were reprogrammed from cardiac fibroblasts rather than dermal fibroblasts or blood mononuclear cells. Here, we continued to investigate the relationship between somatic-cell lineage and hiPSC-CM production by comparing the yield and functional properties of CMs differentiated from iPSCs reprogrammed from human atrial or ventricular cardiac fibroblasts (^AiPSC or ^ViPSC, respectively). Methods: Atrial and ventricular heart tissues were obtained from the same patient, reprogrammed into ^AiPSCs or ^ViPSCs, and then differentiated into CMs (^AiPSC-CMs or ^ViPSC-CMs, re

SUBMITTER: Wang L 

PROVIDER: S-EPMC9950567 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature