Superfluorinated Extracellular Vesicles for In Vivo Imaging by ¹⁹F-MRI.

ABSTRACT: Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and have great potential as efficient delivery vectors. However, a better understanding of EV in vivo behavior is hampered by the limitations of current imaging tools. In addition, chemical labels present the risk of altering the EV membrane features and, thus, in vivo behavior. ¹⁹F-MRI is a safe bioimaging technique providing selective images of exogenous probes. Here, we present the first example of fluorinated EVs containing PERFECTA, a branched molecule with 36 magnetically equivalent ¹⁹F atoms. A PERFECTA emulsion is given to the cells, and PERFECTA-containing EVs are naturally produced. PERFECTA-EVs maintain the physicochemical features, morphology, and biological fingerprint a

SUBMITTER: Sancho-Albero M 

PROVIDER: S-EPMC9951174 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature