Does multi-way, long-range chromatin contact data advance 3D genome reconstruction?

ABSTRACT:

Background

Methods for inferring the three-dimensional (3D) configuration of chromatin from conformation capture assays that provide strictly pairwise interactions, notably Hi-C, utilize the attendant contact matrix as input. More recent assays, in particular split-pool recognition of interactions by tag extension (SPRITE), capture multi-way interactions instead of solely pairwise contacts. These assays yield contacts that straddle appreciably greater genomic distances than Hi-C, in addition to instances of exceptionally high-order chromatin interaction. Such attributes are anticipated to be consequential with respect to 3D genome reconstruction, a task yet to be undertaken with multi-way contact data. However, performing such 3D reconstruction using distance-based reconstruction t

SUBMITTER: Olshen AB 

PROVIDER: S-EPMC9951495 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature